SNARE mediates autophagosome–lysosome fusion
نویسندگان
چکیده
منابع مشابه
The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes.
Endobrevin/VAMP-8 is an R-SNARE localized to endosomes, but it is unknown in which intracellular fusion step it operates. Using subcellular fractionation and quantitative immunogold electron microscopy, we found that endobrevin/VAMP-8 is present on all membranes known to communicate with early endosomes, including the plasma membrane, clathrin-coated pits, late endosomes, and membranes of the t...
متن کاملThe SNARE Ykt6 mediates protein palmitoylation during an early stage of homotypic vacuole fusion.
The NSF homolog Sec18 initiates fusion of yeast vacuoles by disassembling cis-SNARE complexes during priming. Sec18 is also required for palmitoylation of the fusion factor Vac8, although the acylation machinery has not been identified. Here we show that the SNARE Ykt6 mediates Vac8 palmitoylation and acts during a novel subreaction of vacuole fusion. This subreaction is controlled by a Sec17-i...
متن کاملA reduced SNARE model for membrane fusion.
Let's get together: A minimal model system was developed to mimic the SNARE-protein-mediated fusion of biological membranes (see picture). Fusion between two populations of liposomes is controlled by a pair of complementary lipidated oligopeptides that form noncovalent coiled-coil complexes and thereby force the membranes into close proximity to promote fusion. The model system displays the key...
متن کاملVariable cooperativity in SNARE-mediated membrane fusion.
The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex drives the majority of intracellular and exocytic membrane fusion events. Whether and how SNAREs cooperate to mediate fusion has been a subject of intense study, with estimates ranging from a single SNARE complex to 15. Here we show that there is no universally conserved number of SNARE complexes involved ...
متن کاملA lipid-anchored SNARE supports membrane fusion.
Intracellular membrane fusion requires R-SNAREs and Q-SNAREs to assemble into a four-helical parallel coiled-coil, with their hydrophobic anchors spanning the two apposed membranes. Based on the fusion properties of chemically defined SNARE- proteoliposomes, it has been proposed that the assembly of this helical bundle transduces force through the entire bilayer via the transmembrane SNARE anch...
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ژورنال
عنوان ژورنال: Journal of Oral Biosciences
سال: 2012
ISSN: 1349-0079
DOI: 10.1016/j.job.2012.03.001